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b vectastain elite abc hrp kit cat no pk 6100 vector laboratories  (Vector Laboratories)


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    Vector Laboratories b vectastain elite abc hrp kit cat no pk 6100 vector laboratories
    B Vectastain Elite Abc Hrp Kit Cat No Pk 6100 Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elite+abc+vectastain+kit+vector+laboratories+cat/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm42003899-116-3-11
    Average 99 stars, based on 12752 article reviews
    b vectastain elite abc hrp kit cat no pk 6100 vector laboratories - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    Protease Inhibitor:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    Reverse Transcription:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    Control:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    SYBR Green Assay:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    Labeling:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    Enzyme-linked Immunosorbent Assay:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21

    DC Protein Assay:

    Article Title: MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PNGase F New England Biolabs Cat#P0704S Turbofect ThermoFisher Scientific Cat#R0531 Poly-D-Lysine Sigma-Aldrich Cat#P0899 DAPI Sigma-Aldrich Cat#D9542 Triton X-100 Sigma-Aldrich Cat#T9284 ProLong Diamond Antifade Mountant ThermoFisher Scientific Cat#P36965 PEI Transfection Reagent Polysciences Cat#23966–100 Doxycycline Sigma-Aldrich Cat#D3447 PhosSTOP Roche Cat#4906845001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#04693132001 BSA Sigma-Aldrich Cat#A8806 TRI reagent Invitrogen Cat#AM9738 Maxima H Minus Reverse Transcriptase Thermo Fisher Scientific Cat#EP0753 dNTP mix Fermentas UAB Cat#R01936 Trypsin MP Biomedical Cat#103139 HBSS (Ca2+/Mg2+-free) Invitrogen Cat#14170112 poly-DL-ornithine Sigma-Aldrich Cat#P8638 6-OHDA Sigma Chemical CatH-4381 Sodium pentobarbital Orion Pharma N/A Normal horse serum Vector Laboratories Cat#S-2000 3, 30-diaminobenzidine (DAB) Vector Laboratories Cat#SK-4100 Dextran Texas Red Invitrogen Cat#D1864 polyornithine-laminin Sigma-Aldrich Cat#P3655 polyornithine-laminin Sigma-Aldrich Cat#CC095 NGF Promega Cat#G5141 B27 supplement Invitrogen Cat#17504044 Dextran Texas Red Invitrogen Cat#D1864 DharmaFECT reagent Horizon Discovery Cat#T-2001-01 siGENOME Human MANF (7873) siRNA – SMARTpool Horizon Discovery Cat#M-012158-03–0005 siGENOME Non-Targeting siRNA Control Pool #1 Horizon Discovery Cat#D-001206-13-05 Dynabeads Protein G Invitrogen Cat#10004D Critical commercial assays Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Elite ABC Vectastain kit Vector Laboratories Cat#PK-6102 ER-ID Red assay kit Enzo Life Sciences Cat#ENZ-51026-K500 Monolith His-Tag Labeling Kit RED-tris-NTA NanoTemper Technologies GmbH Cat#MO-L008 Duoset ELISA Development System R&D Systems Cat#DY999 mini-PROTEAN precast gels Bio-Rad Cat#456–1093 Phos-tag acrylamide Fujifilm Wako Chemicals Cat#AAL-107S1 Duolink Sigma-Aldrich Cat#DUO92008 DC protein assay (Bio-Rad) Bio-Rad Cat#5000112 Lightcycler 480 SYBR Green I Master mix Roche Diagnostics Cat#04887352001 Experimental models: Cell lines Flp-In T-REx 293 Thermo Fisher Scientific Cat#R78007 Flp-In IRE1a-HA T-REx 293 This study N/A Flp-In BiP-HA T-REx 293 This study N/A (Continued on next page) Cell Reports 42, 112066, February 28, 2023 21



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